@article { WOS:000636357600013, title = {Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor}, journal = {SCIENTIFIC REPORTS}, volume = {11}, year = {2021}, note = {[[{"fid":"256184","view_mode":"default","type":"media","attributes":{"style":"width: 600px; height: 200px;","alt":"zn","class":"img-responsive media-element file-default "}}]] }, month = {MAR 29}, abstract = {

Peptides are commonly used as biosensors for analytes such as metal ions as they have natural binding preferences. In our previous peptide-based impedimetric metal ion biosensors, a monolayer of the peptide was anchored covalently to the electrode. Binding of metal ions resulted in a conformational change of the oxytocin peptide in the monolayer, which was measured using electrochemical impedance spectroscopy. Here, we demonstrate that sensing can be achieved also when the oxytocin is non-covalently integrated into an alkanethiol host monolayer. We show that ion-binding cause morphological changes to the dense host layer, which translates into enhanced impedimetric signals compared to direct covalent assembly strategies. This biosensor proved selective and sensitive for Zn2+ ions in the range of nano- to micro-molar concentrations. This strategy offers an approach to utilize peptide flexibility in monitoring their response to the environment while embedded in a hydrophobic monolayer.


}, issn = {2045-2322}, doi = {10.1038/s41598-021-85015-w}, author = {Attia, Jessica and Nir, Sivan and Mervinetsky, Evgeniy and Balogh, Dora and Gitlin-Domagalska, Agata and Alshanski, Israel and Meital Reches and Mattan Hurevich and Yitzchaik, Shlomo} }