@article {D2OB01886A, title = {Accelerated solid-phase synthesis of glycopeptides containing multiple N-glycosylated sites}, journal = {Org. Biomol. Chem.}, volume = {21}, year = {2022}, note = {}, pages = {1674-1679}, publisher = {The Royal Society of Chemistry}, abstract = {

Peptide fragments of glycoproteins containing multiple N-glycosylated sites are essential biochemical tools not only to investigate protein{\textendash}protein interactions but also to develop glycopeptide-based diagnostics and immunotherapy. However, solid-phase synthesis of glycopeptides containing multiple N-glycosylated sites is hampered by difficult couplings, which results in a substantial drop in yield. To increase the final yield, large amounts of reagents but also time-consuming steps are required. Therefore, we propose herein to utilize heating and stirring in combination with low-loading solid supports to set up an accelerated route to obtain, by an efficient High-Temperature Fast Stirring Peptide Synthesis (HTFS-PS), glycopeptides containing multiple N-glycosylated sites using equimolar excess of the precious glycosylated building blocks.

}, doi = {10.1039/D2OB01886A}, author = {Strauss, Poriah and Nuti, Francesca and Quagliata, Michael and Papini, Anna Maria and Mattan Hurevich} }