@article {3175197, title = {Surface-Controlled Sialoside-Based Biosensing of Viral and Bacterial Neuraminidases}, journal = { Langmuir}, volume = {40}, year = {2024}, note = {

We demonstrate here that the surface interaction adds useful data that are crucial for characterizing protein families that target similar moieties. This new paradigm in array biosensing suggests that in the future, assembling the same set of receptors on a variety of surfaces will enhance and improve the bioinformatics data.

Figure 1

}, pages = {7471{\textendash}7478}, abstract = {

Neuraminidases (NA) are sialic acid-cleaving enzymes that are used by both bacteria and viruses. These enzymes have sialoside structure-related binding and cleaving preferences. Differentiating between these enzymes requires using a large array of hard-to-access sialosides. In this work, we used electrochemical impedimetric biosensing to differentiate among several pathogene-related NAs. We used a limited set of sialosides and tailored the surface properties. Various sialosides were grafted on two different surfaces with unique properties. Electrografting on glassy carbon electrodes provided low-density sialoside-functionalized surfaces with a hydrophobic submonolayer. A two-step assembly on gold electrodes provided a denser sialoside layer on a negatively charged submonolayer. The synthesis of each sialoside required dozens of laborious steps. Utilizing the unique protein{\textendash}electrode interaction modes resulted in richer biodata without increasing the synthetic load. These principles allowed for profiling NAs and determining the efficacy of various antiviral inhibitors.

}, author = {Israel Alshanski and Suraj Toraskar and Daniel Gordon-Levitan and Marco Massetti and Prashant Jain and Luigi Vaccaro and Raghavendra Kikkeri* and Mattan Hurevich* and Shlomo Yitzchaik*} }