Joint with Prof. Shlomo Yitzchaik

Main research topic:Stirred reactor development for solid phase synthesis and electrochemical biosensing of enzymatic processes

Nonscientific Interests: Tennis, music, traveling, chess, detective fiction and sci-fi literature, and movies

Publications:

(1) Sukhran, Y.; Alshanski, I.; Filiba, O.; Mackintosh, M. J.; Schapiro, I.; Hurevich, M.; Unexpected Nucleophile Masking in Acyl Transfer to Sterically Crowded and Conformationally Restricted Galactosides. J. Org. Chem. 2023, In Press. https://doi.org/10.1021/acs.joc.3c00878

(2) Bakhatan, Y.; Alshanski, I.; Chan, C. K.; Lo, W. C.; Lu, P. W.; Liao, P.H.; Wang, C. C.; Hurevich, M.; Accelerated Solid Phase Glycan Synthesis: ASGS. Chem. Eur. J. 2023, 29, e20230089. https://doi.org/10.1002/chem.202300897

(3) Alshanski, I.; Toraskar, S.; Shitrit, A.; Gordon-Levitan, D.; Jain, P.; Kikkeri, R.; Hurevich, M.; Yitzchaik, S.; Biocatalysis versus Molecular Recognition in Sialoside-Selective Neuraminidase Biosensing. ACS Chem. Biol. 2023, 18, 605-614. https://doi.org/10.1021/acschembio.2c00913

(4) Solomon, O.; Alshanski, I.; Shitrit, A.; Chen, Y.J.; Friedler, A.; Yitzchaik, S.; Using a single peptide to electrochemically sense multiple kinases. Biochemistry 2023, 62, 351-357. https://doi.org/10.1021/acs.biochem.2c00411

(5) Shitrit, A.; Mardhekar, S.; Alshanski, I.; Jain, P.; Raigawali, R.; Shanthamurthy, D. C.; Kikkeri, R.; Yitzchaik, S.; Hurevich, M.; Profiling Heparan Sulfate-Heavy Metal Ions Interaction Using Electrochemical Techniques. Chem. Eur. J. 2022, 28, e20220219. https://doi.org/10.1002/chem.202202193 Inside Cover.

(6) Alshanski, I.; Shitrit, A.; Sukhran, Y.; Unverzagt, C.; Hurevich, M.; Yitzchaik, S.; Effect of interfacial properties on impedimetric biosensing of sialylation process with biantennary N-glycan-based monolayer. Langmuir. 2022, 38 (2), 849–855. https://doi.org/10.1021/acs.langmuir.1c02995

(7) Brunori, F.; Kumar Padhi, D.; Alshanski, I.; Freyse, J.; Dürig, N. J.; Penk, A.; Vaccaro, L.; Hurevich, M.; Rademann, J.; Yitzchaik, S. Sulfation pattern dependent Iron (III) mediated interleukin-8 glycan binding. Chembiochem. 2022, 23, e20210055. https://doi.org/10.1002/cbic.202100552.

(8) Naoum, J.#; Alshanski, I.#; Mayer, G. #; Strauss, P.; Hurevich, M. Stirring Peptide Synthesis to a New Level of Efficiency. Org. Process Res. Dev. 2022. 26(1), 137-143 https://doi.org/10.1021/acs.oprd.1c00304

(9) Alshanski, I.; Shalev, D. E.; Yitzchaik, S.; Hurevich, M. Determining the Structure and Binding Mechanism of Oxytocin Cu2+ Complex Using Paramagnetic Relaxation Enhancement NMR Analysis. J. Biol. Inorg. Chem. 2021, 26, 809–815 https://doi.org/10.1007/s00775-021-01897-1.

(10) Alshanski, I.; Sukhran, Y.; Mervinetsky, E.; Unverzagt, C.; Yitzchaik, S.; Hurevich, M. Electrochemical Biosensing Platform Based on Complex Biantennary N-Glycan for Detecting Enzymatic Sialylation Processes. Biosens. Bioelectron. 2021, 172, 112762. https://doi.org/10.1016/j.bios.2020.112762.

(11) Attia, J.; Nir, S.; Mervinetsky, E.; Balogh, D.; Gitlin-Domagalska, A.; Alshanski, I.; Reches, M.; Hurevich, M.; Yitzchaik, S. Non-Covalently Embedded Oxytocin in Alkanethiol Monolayer as Zn2+ Selective Biosensor. Sci. Rep. 2021, 11 (1), 1–7. https://doi.org/10.1038/s41598-021-85015-w.

(12) Dery, S.#; Alshanski, I.#; Mervinetsky, E.; Feferman, D.; Yitzchaik, S.; Hurevich, M.; Gross, E. The Influence of Surface Proximity on Photoswitching Activity of Stilbene-Functionalized N-Heterocyclic Carbene Monolayers. Chem. Commun. 2021, 57 (51), 6233–6236. https://doi.org/10.1039/d1cc02491d. Inside Cover, Featured in chemical communications HOT articles.

(13) Bakhatan, Y.; Alshanski, I.; Grunhaus, D.; Hurevich, M. The Breaking Beads Approach for Photocleavage from Solid Support. Org. Biomol. Chem. 2020, 18 (22), 4183–4188. https://doi.org/10.1039/d0ob00821d.

(14) Mervinetsky, E.#; Alshanski, I.#; Tadi, K. K.; Dianat, A.; Buchwald, J.; Gutierrez, R.; Cuniberti, G.; Hurevich, M.; Yitzchaik, S. A Zinc Selective Oxytocin Based Biosensor. J. Mater. Chem. B 2020, 8 (1), 155–160. https://doi.org/10.1039/c9tb01932d.

(15) Mervinetsky, E.; Alshanski, I.; Lenfant, S.; Guerin, D.; Medrano Sandonas, L.; Dianat, A.; Gutierrez, R.; Cuniberti, G.; Hurevich, M.; Yitzchaik, S.; Vuillaume, D. Electron Transport through Self-Assembled Monolayers of Tripeptides. J. Phys. Chem. C 2019, 123 (14), 9600–9608. https://doi.org/10.1021/acs.jpcc.9b01082.

(16) Synhaivska, O.; Mermoud, Y.; Baghernejad, M.; Alshanski, I.; Hurevich, M.; Yitzchaik, S.; Wipf, M.; Calame, M. Detection of Cu2+ Ions with GGH Peptide Realized with Si-Nanoribbon ISFET. Sensors 2019, 19 (18), 4022. https://doi.org/10.3390/s19184022.

(17) Naoum, J. #; Alshanski, I.#; Gitlin-Domagalska, A.; Bentolila, M.; Gilon, C.; Hurevich, M. Diffusion Enhanced Amide Bond Formation on Solid Support. Org. Process Res. Dev. 2019, 23, 2733–2739. https://doi.org/10.1021/acs.oprd.9b00398.

(18) Mervinetsky, E.; Alshanski, I.; Buchwald, J.; Dianat, A.; Lončarić, I.; Lazić, P.; Crljen, Ž.; Gutierrez, R.; Cuniberti, G.; Hurevich, M.; Yitzchaik, S. Direct Assembly and Metal - Ions Binding Properties of Oxytocin Monolayer on Gold Surfaces. Langmuir 2019, 35 (34), 11114–11122. https://doi.org/10.1021/acs.langmuir.9b01830. Inside Cover.

(19) Alshanski, I.; Blaszkiewicz, J.; Mervinetsky, E.; Rademann, J.; Yitzchaik, S.; Hurevich, M. Sulfation Patterns of Saccharides and Heavy Metal Ions Binding. Chem. Eur. J. 2019, 25 (52), 12083–12090. https://doi.org/10.1002/chem.201901538. Inside Cover.

(20) Gankin, A.; Mervinetsky, E.; Alshanski, I.; Buchwald, J.; Dianat, A.; Gutierrez, R.; Cuniberti, G.; Sfez, R.; Yitzchaik, S. ITO Work Function Tunability by Polarizable Chromophore Monolayers. Langmuir 2019, 35, 2997–3004. https://doi.org/10.1021/acs.langmuir.8b03943.

(21) Tadi, K. K.; Alshanski, I.; Hurevich, M.; Yitzchaik, S. PH Controlled Impedimetric Sensing of Copper (II) Ion Using Oxytocin as Recognition Element. Surfaces 2018, 1 (1), 90–95. https://doi.org/10.3390/surfaces1010008.

(22) Samarasimhareddy, M.; Alshanski, I.; Mervinetsky, E.; Hurevich, M.; Photodeprotection of up to Eight Photolabile Protecting Groups from a Single Glycan. Synlett 2018, 29 (07), 880–884. https://doi.org/10.1055/s-0036-1591915.

(23) Alshanski, I.; Bentolila, M.; Gitlin-Domagalska, A.; Zamir, D.; Zorsky, S.; Joubran, S.; Hurevich, M.; Gilon, C. Enhancing the Efficiency of the Solid Phase Peptide Synthesis (SPPS) Process by High Shear Mixing. Org. Process Res. Dev. 2018, 22, 1318–1322. https://doi.org/10.1021/acs.oprd.8b00225.

(24) Bentolila, M.; Alshanski, I.; Novoa, R.; Gilon, C. Optimization of Chemical Processes by the Hydrodynamic Simulation Method (HSM). Chemengineering 2018, 2 (2), 21. https://doi.org/10.3390/chemengineering2020021.

(25) Mervinetsky, E.; Alshanski, I.; Hamo, Y.; Sandonas, L. M.; Dianat, A.; Buchwald, J.; Gutierrez, R.; Cuniberti, G.; Hurevich, M.; Yitzchaik, S. Copper Induced Conformational Changes of Tripeptide Monolayer Based Impedimetric Biosensor. Sci. Rep. 2017, 7 (1), 1–7. https://doi.org/10.1038/s41598-017-10288-z.

(26) Tadi, K. K.; Alshanski, I.; Mervinetsky, E.; Marx, G.; Petrou, P.; Dimitrios, K. M.; Gilon, C.; Hurevich, M.; Yitzchaik, S. Oxytocin-Monolayer-Based Impedimetric Biosensor for Zinc and Copper Ions. ACS Omega 2017, 2 (12), 8770–8778. https://doi.org/10.1021/acsomega.7b01404.

(27) Amiel, D. B. A.; Alshanski, I.; Hurevich, M. Automated Oligosaccharide Synthesis: Development of the Glyconeer®. Comprehensive Glycoscience, 2nd ed.; Elsevier B.V., 2021, 548-560. https://doi.org/10.1016/b978-0-12-819475-1.00069-9

Yasmeen was born and raised in Jerusalem. She completed her B.Sc.in Chemistry ,Birzeit University. and M.Sc. in chemistry in 2020 ,The Hebrew University of Jerusalem. Her Current research is synthesis of a library of chitosan using Automated Glycan Assembly and automatic synthesis glycopeptides. Her hobbies include reading and playing tennis

Publications:

• The Breaking Beads Approach for Photocleavage from Solid Support.  Bakhatan et al. Org Biomol Chem,2020

B.Sc in the joint program for chemistry and biology, The Hebrew University of Jerusalem.

M. Sc student for Chemistry . 

Main research topic:synthesis of proteoglycan core glycan

Hobbies:Basktball, Tennis, Creative writing, D&D, Fountain pens and Inks, home-brewing, restorations

B.Sc in joint ptogram of Chemistry and Biology and Amirim-Science(2020-2023).

M.Sc student in nanoscience and nanotechnolegy program.

Main research topic: Caged Phosphorelated Peptides

Nonscientific Interests:Music,dancing ballet and reading books

Publication:D. Grunhaus, E. Rossich Molina, R. Cohen, T. Stein, A. Friedler
and M. Hurevich, Org. Proc. Res. Dev., 2022, 26, 2492–2497.

Joint with Prof Assaf Friedler.

B.Sc. in Chemistry and Biology The Hebrew University of Jerusalem, Israel. M.Sc in Chemistry The Hebrew University of Jerusalem, Israel.

The topic of my research is new methods for synthesis of peptides with post-translational modifications and their effect in biological systems.
Joint with Prof Assaf Friedler

1.  Grunhaus D., Friedler A., Hurevich M., (2021) Automated synthesis of heavily phosphorylated peptides.  Eur Joc. https://doi.org/10.1002/ejoc.202100691

2.  Bakhatan Y, Alshanski I, Grunhaus D, and Hurevich M (2020) The breaking beads approach for photocleavage from solid support. Org Biomol Chem 18:4183–4188. https://doi.org/10.1039/d0ob00821d

3.  Faust O., Grunhaus D., Shimshon O., Yavin E., and Friedler A. (2018) Protein Regulation by Intrinsically Disordered Regions: A Role for Subdomains in the IDR of the HIV-1 Rev Protein. Chembiochem 19:1618-1624 https://doi.org/10.1002/cbic.201800192

4. Grunhaus D., Estefanía Rossich M., Cohen R., Stein T., Friedler A.*, Hurevich M.* (2022) Accelerated Multiphosphorylated Peptide Synthesis, Org. Process Res. Dev. 2022, 26, 8, 2492–2497https://doi.org/10.1021/acs.oprd.2c00164

B.Sc and M.Sc in Biochemistry and food science at the faculty of agriculture, food and environment of the Hebrew University at Rehovot. Currently, pHD student in collaboration with Prof. Zvi Hayouka from the agriculture faculty. My research topic is development of biosensors for bacteria detection based on peptides as recognition molecules.

1.      Stern T., Zelinger E. and Hayouka Z. 2016. Random peptide mixtures inhibit and eradicate methicillin-resistant Staphylococcus aureus biofilms.  Chemical Communications, 7102-7105

2.      Ryadnov M., Hayouka Z., Bella A., Stern T., Jiang H., Ray S., & Grovenor C. 2017. Binary encoding of peptide sequences into differential antimicrobial mechanisms. Angewandte Chemie, 8211-8215

3.      Topman, S., Tamir‐Ariel, D., Bochnic‐Tamir, H., Stern Bauer, T., Shafir, S., Burdman, S., & Hayouka, Z. 2018. Random peptide mixtures as new crop protection agents. Microbial biotechnology, 1027-1036.‏

4.      Stern Bauer, T. and Hayouka, Z. 2018. Random mixtures of antimicrobial peptides inhibit bacteria associated with pasteurized bovine milk. Journal of Peptide Science, e3088.‏

5.     Stern Bauer, T, Menagen, B., Avnir, D., & Hayouka, Z. 2019. Random peptide mixtures entrapped within a copper-cuprite matrix: new antimicrobial agent against methicillin-resistant Staphylococcus aureus. Scientific reports, 9(1), 1-8.‏

6.     Keshri, J., Krouptiski, Y., Abu-Fani, L., Achmon, Y., Stern Bauer, T.., Zarka, O., Maler, I., Pinto, R., & Saldinger, S. S. 2019. Dynamics of bacterial communities in alfalfa and mung bean sprouts during refrigerated conditions. Food microbiology, 84, 103261.‏

Completed  B.Sc. double major in Chemistry and Life Sciences at The Hebrew University of Jerusalem in 2021, and  M.Sc. in Organic Chemistry at The Hebrew University of Jerusalem in 2023.

 Main Current research topic: solid phase synthesis of a library of sulfated carrageenans.

Nonscientific Interests: Woodworking and origami.

Publications:

(1) Sukhran, Y.; Alshanski, I.; Filiba, O.; Mackintosh, M. J.; Schapiro, I.; Hurevich, M.; Unexpected Nucleophile Masking in Acyl Transfer to Sterically Crowded and Conformationally Restricted Galactosides. J. Org. Chem. 2023,88 (13),9313-9320. https://doi.org/10.1021/acs.joc.3c00878.

(2) Bakhatan, Y.; Ben Abba Amiel, D.; Sukhran, Y.; Chan, C. K.; Lo, W. C.; Lu, P. W.; Liao, P. H.; Wang, C. C.; Hurevich, M. Translating Solution to Solid Phase Glycosylation Conditions. Chem. Commun. 2022, 58 (80), 11256–11259. https://doi.org/10.1039/D2CC03831E.

(3)Alshanski, I.; Shitrit, A.; Sukhran, Y.; Unverzagt, C.; Hurevich, M.; Yitzchaik, S.; Effect of interfacial properties on impedimetric biosensing of sialylation process with biantennary N-glycan-based monolayer. Langmuir. 2022, 38 (2), 849–855. https://doi.org/10.1021/acs.langmuir.1c02995.

(4) Alshanski, I.; Sukhran, Y.; Mervinetsky, E.; Unverzagt, C.; Yitzchaik, S.; Hurevich, M. Electrochemical Biosensing Platform Based on Complex Biantennary N-Glycan for Detecting Enzymatic Sialylation Processes. Biosens. Bioelectron. 2021, 172, 112762. https://doi.org/10.1016/j.bios.2020.112762.

Dror was born and lives in Jerusalem. He is married, and is a fan of basketball, hiking, nature photography and cooking. Academic career: B.Sc, 2016, Joint program for physics and chemistry, Hebrew University of Jerusalem; M.Sc, 2019, Hebrew University of Jerusalem. His Current research concerns synthesis of glycopeptide libraries.

Publications:

(1) Ben Abba Amiel, D.; Kim, C.; Gidron, O. Oligofuran–Benzothiadiazole Co-Oligomers: Synthesis, Optoelectronic Properties and Reactivity. Org. Mater. 2021, 03 (02), 303–308. https://doi.org/10.1055/s-0041-1729853.

(2) Ben Abba Amiel, D.; Alshanski, I.; Hurevich, M. Automated Oligosaccharide Synthesis: Development of the Glyconeer®. Compr. Glycosci. 2021, 548–560. https://doi.org/10.1016/B978-0-12-819475-1.00069-9

 B.Sc student majoring in chemistry with a minor in biochemistry at The Hebrew University of Jerusalem.

Main research topic: Glycosilation optimization and BB synthesis

Hobbies including reading, gardening, photography, calligraphy, playing guitar, winemaking, aviation, bookbinding, electronics, knitting and sewing

B.Sc in chemistry, Universidad del Valle de México, México, D.F.

M.Sc in chemistry. 

Main research topic: N-Glycans 

Hobbies:Theater

B.Sc student in chemistry. 

Main research topic: application of proteglycam-coglycan methology.

Hobbies:EMT and scuba diving

Publications: 

1. Application of Ambr15 system for simulation of entire SARS-CoV-2 vaccine production process involving macrocarriers 'Biotechnology Progress"

2. Novel method for quantifying cells on carriers and its demonstration during SARS-2 vaccine development 'biotechnology and bioengineering'

3. A simple method for in situ qutification of cells on carriers 'Bio-protocol'

4. In-Line Monitoring of Downstream Purification Processes for VSV Based SARS-CoV-2 Vaccine Using a Novel Technique 'biotech'

5. Poly(vinyl alcohol)-methacrylate with CRDG peptide: A photocurable biocompatible hydrogel 'journal of regenerative medicine and tissue engineering'

6. SARS-CoV-2 spike antigen quantification by targeted mass spectrometry of a virus-based vaccine 'Journal of Virological Methods'

7. Optimization of VSV-ΔG-spike production process with the Ambr15 system for a SARS-COV-2 vaccine 'biotechnology and bioengineering'

8. PEG-fibrinogen hydrogel microspheres as a scaffold for therapeutic delivery of immune cells 'Frontiers in Bioengineering and Biotechnology'

B.sc in biochemistry and food science.

Main research topic: glycan&peptide based bio-sensors

Hobbies:Reading, Cooking, baking, solid state fermentation